Design and fabrication of fluidic devices for industrial-scale production of lipid-based nanoparticles and biomolecules encapsulation

Abstract

Liposome nanoparticles are produced using top-down and bottom-up technics, and they are used as delivery carriers in drug delivery, proteins, labels, and genetic material applications. Increasing liposome throughput and reproducibility are essential for industrial-scale production of liposomes in order to consider them as nanomedicines that could be possible using micromixers as well as milli mixers. Micrometer-scale mixers (micromixers) generate nanoprecipitation which is one of the most successful bottom-up approaches and can control properties of liposomes such as their size and size distribution. On the other hand, millimeterscale microchannels (milli mixers) are able to produce liposomes of nanometric scale, by which the production of liposomes at high rates is allowed because of the larger cross-section dimension. Besides, the reducing of toxic residues is another challenge related to the mentioned approach. In this work, different designs for producing fluidic devices are explored using numerical simulation and modern fabrication techniques. Controlling liposomes size, size distribution, and zeta potential (ZP), as well as their toxicity, purity and production rate, is done by characterizing and evaluating synthesized liposomes.

Date	26 Nov 2024
Original language	American English
Awarding Institution	École de technologie supérieure
Supervisor	Vahé Nerguizian (Supervisor) & Ion Stiharu (Co-supervisor)