Traitement des eaux usées municipales par phanerochaete chrysosporium

Abstract

The presence of many recalcitrant micro-pollutants identified in the effluent discharge because of concerns increasingly growing. Although there are various methods of treatment, those are not economically viable. In this context, this article aimed to develop a biotechnology strategy (mycoremediation) economically viable, environmentally friendly and efficient to ensure sanitation for domestic wastewater and removal of micropollutants. This relatively new technique focus on the ability of fungi to degrade a wide range of pollutants through the enzymes they secrete. In this study, the fungus white rot Phanerochaete chrysosporium was used for the manufacture of grafts to produce an acceptable quality of effluent prior to discharge into the receiving environment. The first part of the experimental protocol was to cultivate Phanerochaete chrysosporium on several media of different shapes and materials. Polyurethane and polyethylene showed a better growth. However, the choice fell on the pellets of Degrémont because polyurethane and polyethylene were too large. The second part has analyzed the fungus treatment effectiveness on wastewaters through several tests (COD, BOD 5, TSS, Phosphorus, nitrogen etc.) and under different culture conditions (pH, temperature etc.). It appears at the end of this experience that the treated areas with grafts show a better quality than the treated areas only with Phanerochaete chrysosporium. The third part focused on quantifying the enzymatic production (MnP and LiP) with and without carrier to observe the effect of the media on this production.The results indicate a reduction of COD and BOD5 respectively 98 % and 97 % and lower 44 % TSS is also observed. These results also indicate that the pellets would improve the enzyme production and they also have the ability to oppose the change in pH. Screening to determine the limits of the process indicates that 50 to 75 grafts, pH 4.5 to 5.5 and a temperature of 25 ° C to 35 ° C are necessary for a better fungal treatment. Above and below these numbers, the treatment was not as effective.

Date	22 Nov 2016
Original language	French
Awarding Institution	École de technologie supérieure
Supervisor	Robert Hausler (Supervisor)